T10 lateral hemisection spinal cord injury with multiple histological and behavioral outcomes

DOI:10.7295/W9HQ3X20

DATASET CITATION

Yuanyuan Liu, Xuhua Wang, Wenlei Li, Qian Zhang, Yi Li, Zicong Zhang, Junjie Zhu, Bo Chen, Philip R. Williams, Yiming Zhang, Bin Yu, Xiaosong Gu, Zhigang He (2019). T10 lateral hemisection spinal cord injury with multiple histological and behavioral outcomes. Open Data Commons for Spinal Cord Injury. ODC-SCI:212 http://doi.org/10.7295/W9HQ3X20

ABSTRACT

STUDY PURPOSE: A major hurdle for functional recovery after spinal cord injury is the limited re- growth of the axons in the corticospinal tract (CST) that originate in the motor cortex and innervate the spinal cord. In this study, we tested whether post-lesional AAV-assisted co- expression of two soluble proteins, namely insulin-like growth factor 1 (IGF1) and osteopontin (OPN), in cortical neurons leads to CST regrowth and CST-dependent functional recovery.

DATA COLLECTED: With an incomplete spinal cord injury model (T10 lateral hemisection), we tested the sensitizing effect of OPN/IGF1 on corticospinal neurons, the extent of axon regrowth both in the injured side (axon regeneration) and the intact side (collateral axon sprouting). In addition, we assessed behavioral performance for both gross and skilled locomotion in mice treated with OPN/IGF1 post T10 lateral hemisection.

PRIMARY CONCLUSION: Our results demonstrate a potentially translatable strategy for restoring cortical dependent function after injury in the adult.

KEYWORDS

Osteopontin (OPN); Insulin like growth factor 1 (IGF1); axon regeneration; axon sprouting; functional recovery

PROVENANCE / ORIGINATING PUBLICATIONS

• Yuanyuan Liu, Xuhua Wang, Wenlei Li, Qian Zhang, Yi Li, Zicong Zhang, Junjie Zhu, Bo Chen, Philip R. Williams, Yiming Zhang, Bin Yu, Xiaosong Gu, Zhigang He (2017) A Sensitized IGF1 Treatment Restores Corticospinal Axon-Dependent Functions. Neuron 4(95). 817-833.e4. DOI:10.1016/j.neuron.2017.07.037.

Primary data collected and analyzed in this paper are all reported in Liu et al., Neuron, 2017.

DATASET INFO

Contact: Zhigang He (Zhigang.He@childrens.harvard.edu)

Lab: He Lab

Records in Dataset: 5217

Last updated: 2019-08-19 (See changelog)

Date published: 2019-08-19

Downloads: 21

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

National Institutes of Health grants P30HD018655 and P30EY012196; National Institute of Neurological Disorders and Stroke (NS096294); Craig Neilsen Foundation (Y. Liu, Y. Li, and X.W.); Wings for Life; Hong Kong Spinal Cord Injury Fund, and the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (to Z.H.).

CONTRIBUTORS

Yuanyuan Liu [ORCID: 0000-0001-9865-1378]

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Xuhua Wang

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Wenlei Li

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Qian Zhang

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Yi Li

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Zicong Zhang [ORCID: 0000-0002-6453-5528]

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Junjie Zhu [ORCID: 0000-0002-3827-1741]

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Bo Chen

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Philip R. Williams [ORCID: 0000-0003-4813-8925]

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Yiming Zhang

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Bin Yu

Key laboratory of neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, China 226001

Xiaosong Gu

Key laboratory of neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, China 226001

Zhigang He

F.M. Kirby Neurobiology Center, Boston Children’s Hospital, and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA, 02115, USA

Almeida, Carlos [ORCID: 0000-0001-9997-8719]

University of California San Francisco (UCSF), Brain and Spinal Injury Center, Dept Neurosurgery, 1001 Potrero Ave, 1001 Potrero Ave, San Francisco, California, United States, 94110

Abel Torres-Espin [ORCID: 0000-0002-9787-8738]

University of California San Francisco (UCSF), Brain and Spinal Injury Center, Dept Neurosurgery, 1001 Potrero Ave, 1001 Potrero Ave, San Francisco, California, United States, 94110