Data for the manuscript: Fecal Transplant Prevents Gut Dysbiosis and Anxiety-like Behaviour After Spinal Cord Injury in Rats

DOI:10.7295/W97942VQ

DATASET CITATION

Schmidt, E., Torres-Espin, A., Raposo, P., Madsen, K., Kigerl, K., Popovich., P., Fenrich, K.F., Fouad, K. (2020) Data for Fecal Transplant Prevents Gut Dysbiosis and Anxiety-like Behaviour After Spinal Cord Injury in Rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:262 http://doi.org/10.7295/W97942VQ

ABSTRACT

STUDY PURPOSE: To establish a model of anxiety following a cervical contusion spinal cord injury (SCI) in rats and to determine whether the microbiota play a role in the observed behavioural changes.

DATA COLLECTED: This dataset includes n=57 rats from 2 experiments. Experiment 1: sham (underwent surgery with no SCI) n=6; unilateral cervical contusion SCI n=6. Experiment 2: Healthy (no operation, no gavage) n=10; Sham n=11; SCI (gavaged with a control solution) n=10; SCI-FMT (gavaged with fecal microbiota transplant solution) n=14. A subset of subjects from experiment 2 were used for the fecal 16s rRNA analysis (healthy n = 10; SCI-FMT n = 10; sham n = 5; SCI n = 5). Fecal matter for the 16s rRNA analysis were collected before injury, 3 days after injury and 4 weeks after injury. A PICRUST analysis was performed to infer the functional pathways involved using the 16s rRNA gene data. Rats were assessed on a battery of behavioural tests: the light-dark box, the cylinder test, the sucrose preference test, the elevated plus maze and the open field. Lesion size was calculated as the percentage of damaged tissue area throughout the rostral-caudal extension of the injury site. 

PRIMARY CONCLUSION: Treatment with a fecal microbiota transplant in the acute post-injury period prevents spinal cord injury-induced gut dysbiosis as well as the development of anxiety-like behaviour.   

KEYWORDS

Spinal Cord Injury; Anxiety; Dysbiosis; Fecal Microbiota Transplant

PROVENANCE / ORIGINATING PUBLICATIONS

Schmidt, E., Torres-Espin, A., Raposo, P., Madsen, K., Kigerl, K., Popovich., P., Fenrich, K.F., Fouad, K. (2019) Fecal Transplant Prevents Gut Dysbiosis and Anxiety-like Behaviour After Spinal Cord Injury in Rats. PLOS One. January 15, 2020 doi.org/10.1371/journal.pone.0226128

• This publication serves as the originating work for the presented data

DATASET INFO

Contact: Karim Fouad (karim.fouad@ualberta.ca)

Lab: Fouad Lab

ODC-SCI Accession: 262

Records in Dataset: 58770

Fields per Record: 39

Last updated: 2020-12-03 (See changelog)

Date published: 2020-12-03

Downloads: 11

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

Craig Neilsen Foundation (NPRG 542589); Canadian Institutes of Health Research (MOP 119281)

CONTRIBUTORS

Schmidt, Emma [ORCID: 0000-0001-9803-6391]

Faculty of Rehabilitation Medicine, Department of Physical Therapy, Neuroscience and Mental Health Institute, University of Alberta; Canada

Torres-Espin, Abel [ORCID: 0000-0002-9787-8738]

Faculty of Rehabilitation Medicine, Department of Physical Therapy, Neuroscience and Mental Health Institute, University of Alberta; Canada

Fouad, Karim [ORCID: 0000-0003-3654-7852]

Faculty of Rehabilitation Medicine, Department of Physical Therapy, Neuroscience and Mental Health Institute, University of Alberta; Canada

Raposo, Pamela

Faculty of Rehabilitation Medicine, Department of Physical Therapy, Neuroscience and Mental Health Institute, University of Alberta; Canada

Fenrich, Keith [ORCID: 0000-0003-4360-064X]

Faculty of Rehabilitation Medicine, Department of Physical Therapy, Neuroscience and Mental Health Institute, University of Alberta; Canada

Madsen, Karen [ORCID: 0000-0002-8636-0714]

Division of Gastroenterology, University of Alberta; Canada

Kigerl, Kristina [ORCID: 0000-0002-1652-0882]

Department of Neuroscience, Center for Brain and Spinal Cord Repair, The Belford Center for Spinal Cord Injury, The Ohio State University Wexner Medical Center; USA

Popovich, Phil [ORCID: 0000-0003-1329-7395]

Department of Neuroscience, Center for Brain and Spinal Cord Repair, The Belford Center for Spinal Cord Injury, The Ohio State University Wexner Medical Center; USA