Data for manuscript: Delayed short-term tamoxifen treatment does not promote remyelination or neuron sparing after spinal cord injury

DATASET CITATION

Puko, N., McTigue, D.M. (2020). Data for manuscript: Delayed short-term tamoxifen treatment does not promote remyelination or neuron sparing after spinal cord injury. Open Data Commons for Spinal Cord Injury. ODC-SCI:419 http://dx.doi.org/10.34945/F5QP4H

ABSTRACT

STUDY PURPOSE: Tamoxifen is widely used to spatially and temporally control gene expression; however, whether tamoxifen has off-target effects remains elusive. Here, we tested whether delayed short-term tamoxifen treatment changed motor and/or anatomical recovery after spinal cord injury.

DATA COLLECTED: This dataset includes n = 40 adult C57BL/6J mice (n = 20 female, n = 20 male) that received a moderate (75 kDyne) spinal cord contusion injury. Tamoxifen or corn-oil was gavaged 19-22 days post-injury and mice were sacrificed 42 days post-injury. Motor recovery was assessed using the Basso Mouse Scale, automated horizontal ladder, and activity box. Anatomical recovery was analyzed via white matter sparing (Eriochrome Cyanine), neuron survival (NeuN), axon sparing (NF-H) and regeneration (GAP-43), astrocyte reactivity (GFAP), lesion size (GFAP), cell proliferation (EDU; NG2/EDU; CD68/NG2), and changes in myelination (nodes of Ranvier; Caspr/Kv1.2).

PRIMARY CONCLUSION: Delayed short-term tamoxifen gavage does not alter motor recovery, neuroprotection, or neurorestoration after spinal cord injury.

KEYWORDS

Spinal Cord Injury, Myelination, Tamoxifen