Minocycline treatment for acute cervical spinal cord injury in female rats: microbiota composition
DOI:10.34945/F5F30N
DATASET CITATION
Schmidt E. K A., Raposo P. J F., Torres-Espin A., Fenrich K. K., Fouad K. (2021) Minocycline treatment for acute cervical spinal cord injury in female rats: microbiota composition. Open Data Commons for Spinal Cord Injury. ODC-SCI: 454 http://doi.org/10.34945/F5F30N
ABSTRACT
STUDY PURPOSE: Minocycline has been widely studied for its local anti-inflammatory properties; however little is known about its antibiotic and systemic immune effects following spinal cord injury (SCI). The aim of the present study is to elucidate multiple system-wide changes involving the microbiota-immune axis induced by minocycline treatment in a rodent model of cervical contusion SCI (unilateral contusion 125 kdyns at an angle of 15 degrees to the right at C5). This study included 4 groups of adult female Lewis rats: uninjured n=10, uninjured + minocycline n=10, SCI n=8, SCI + minocycline n=9. Minocycline (or sterile water for untreated control groups) was administered at a dose of 50mg/kg via oral gavage for 7 days immediately following injury.
DATA COLLECTED: fecal microbiota data: 16s rRNA sequencing data (at the phylum, class, order, family, genus and species taxonomic levels) from fecal samples collected before injury, on the day of injury, 5, 14 and 28 days post injury
DATA USAGE NOTES: The present data shows that 7 days of minocycline treatment had a drastic acute effect on the microbiota composition regardless of whether the animal was uninjured or had a SCI. Later effects of SCI vs. uninjured rats emerged by 28 days post-SCI.
KEYWORDS
Spinal Cord Injury; minocycline; 16S rRNA
PROVENANCE / ORIGINATING PUBLICATIONS
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DATASET INFO
Contact: Fouad Karim (karim.fouad@ualberta.ca)
Lab: Karim Fouad
ODC-SCI Accession:454
Records in Dataset: 180
Fields per Record: 261
Last updated: 2021-01-22
Date published: 2021-01-22
Downloads: 16
Files: 2
LICENSE
Creative Commons Attribution License (CC-BY 4.0)
FUNDING AND ACKNOWLEDGEMENTS
Craig Neilsen Foundation NPRG 542589 (KF)
CONTRIBUTORS
- Schmidt, Emma K A. [ORCID:0000-0001-9803-6391]
- Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada
- Raposo, Pamela J F. [ORCID:0000-0001-6350-5223]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
- Torres-Espin, Abel [ORCID:0000-0002-9787-8738]
- University of California San Francisco, Brain and Spinal Injury Center, Dept. of Neurological Surgery; San Francisco, California, USA
- Fenrich, Keith K. [ORCID:0000-0003-4360-064X]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
- Fouad, Karim [ORCID:0000-0003-3654-7852]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
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