Minocycline treatment for acute cervical spinal cord injury in female rats: behavioural, histopathological and systemic immune effects
DOI:10.34945/F5JS3M
DATASET CITATION
Schmidt E. K A., Raposo P. J F., Torres-Espin A., Fenrich K. K., Fouad K. (2021) Minocycline treatment for acute cervical spinal cord injury in female rats: behavioural, histopathological and systemic immune effects. Open Data Commons for Spinal Cord Injury. ODC-SCI: 457 http://doi.org/10.34945/F5JS3M
ABSTRACT
STUDY PURPOSE: Minocycline has been widely studied for its local anti-inflammatory properties; however little is known about its antibiotic and systemic immune effects following spinal cord injury (SCI). The aim of the present study is to elucidate multiple system-wide changes involving the microbiota-immune axis induced my minocycline treatment in a rodent model of cervical contusion SCI (unilateral contusion 125 kdyns at an angle of 15 degrees to the right at C5). This study included 4 groups of adult female Lewis rats: uninjured n=10, uninjured + minocycline n=10, SCI n=8, SCI + minocycline n=9. Minocycline (or sterile water for untreated control groups) was administered at a dose of 50mg/kg via oral gavage for 7 days immediately following injury.
DATA COLLECTED: pICRUST bioinformatics software was used to predict the metagenomic functional content of the 16s rRNA data; behavioural data from the open field and cylinder tasks, anxiety- and depressive-like behaviour was analyzed in the elevated plus maze, light/dark box and sucrose preference tests; quantification of the lesion area and extension; 29 plasma analytes (cytokines, chemokines and hormones) were measured before injury, 5, 14, and 28 days post injury; quantification of microglia (iba1) immunoreactivity above, at and below the lesion site; body weight monitored weekly throughout the experiment. 16s rRNA sequencing data from fecal samples collected before injury, on the day of injury, 5, 14 and 28 days post injury is located in a separate dataset.
DATA USAGE NOTES: The present data shows that 7 days of minocycline treatment had a drastic acute effect on the microbiota composition regardless of whether the animal was uninjured or had a SCI. SCI resulted in a delayed (measured 28 days after injury) suppression of inflammatory cytokines/chemokines in the plasma, which was prevented with minocycline treatment. Minocycline treatment had a modest but insignificant anxiolytic effect in the light/dark box following SCI. Finally, we did not find minocycline treatment to attenuate lesion size or extension, however there was an increase in iba1 immunoreactivity above and below the lesion site in the minocycline + SCI group compared to untreated SCI rats.
KEYWORDS
minocycline; Spinal Cord Injury; Inflammation
PROVENANCE / ORIGINATING PUBLICATIONS
|
|
DATASET INFO
Contact: Fouad Karim (karim.fouad@ualberta.ca)
Lab: Karim Fouad
ODC-SCI Accession:457
Records in Dataset: 288
Fields per Record: 94
Last updated: 2021-01-22
Date published: 2021-01-22
Downloads: 17
Files: 2
LICENSE
Creative Commons Attribution License (CC-BY 4.0)
FUNDING AND ACKNOWLEDGEMENTS
Craig Neilsen Foundation NPRG 542589 (KF)
CONTRIBUTORS
- Schmidt, Emma K A. [ORCID:0000-0001-9803-6391]
- Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada
- Raposo, Pamela J F. [ORCID:0000-0001-6350-5223]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
- Torres-Espin, Abel [ORCID:0000-0002-9787-8738]
- University of California san Fransisco, Brain and Spinal Injury Center, dept. of Neurological Surgery; San Francisco, California, USA
- Fenrich, Keith K. [ORCID:0000-0003-4360-064X]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
- Fouad, Karim [ORCID:0000-0003-3654-7852]
- Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada
|
