Histological measurement of interstitial fluid flow and relationship to lesion volume after C4 hemicontusion in male and female rats

DOI:10.34945/F54016

DATASET CITATION

Kwon H. Y., Cornelison C. (2024) Histological measurement of interstitial fluid flow and relationship to lesion volume after C4 hemicontusion in male and female rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:1121 http://doi.org/10.34945/F54016

ABSTRACT

STUDY PURPOSE: To measure the effects of spinal cord injury on interstitial pressure and fluid flow and examine the potential role of interstitial fluid flow on lesion volume enlargement.

DATA COLLECTED: We collected interstitial pressure measurements at 1 hr, 3 days, and 7 days after contusion SCI in Sprague Dawley rats. We also used histological assessments to determine the distance of Evans Blue dye extravasation at days 3 and 7 after injury (proxy for interstitial fluid flow distance). Finally, we exogenously enhanced interstitial fluid flow using convection enhanced delivery at 7 days after injury and measured the resulting lesion volume compared to untreated controls.

CONCLUSIONS: Spinal interstitial pressure increases from -3 mmHg in the naive cord to a peak of 13 mmHg at 3 days post-injury (DPI) but relatively normalizes to 2 mmHg by 7 DPI. By quantifying vascular leakage of Evans Blue dye after a cervical hemi-contusion in rats, we confirm an increase in dye infiltration at 3 DPI compared to 7 DPI, suggestive of higher fluid velocities at the time of peak fluid pressure. Finally, exogenously enhancing interstitial flow significantly increases the resulting lesion volume.

KEYWORDS

interstitial fluid; fluid transport; histological dye; cavity

PROVENANCE / ORIGINATING PUBLICATIONS

• Convective forces contribute to post-traumatic degeneration after spinal cord injury Hoi Y Kwon, Christopher Streilein, R. Chase Cornelison bioRxiv 2024.06.17.599340. doi:10.1101/2024.06.17.599340.

Preprinted paper containing the present data

RELEVANT LINKS

NOTES

DATASET INFO

Contact: Cornelison Chase (rcornelison@umass.edu)

Lab: Cornelison Group

ODC-SCI Accession:1121

Records in Dataset: 38

Fields per Record: 32

Last updated: 2024-09-04

Date published: 2024-09-04

Downloads: 6

Files: 3

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

University of Massachusetts Amherst Start-up funds (RCC)

CONTRIBUTORS

Kwon, Hoi Y.

Department of Chemical Engineering, University of Massachusetts Amherst

Cornelison, Chase [ORCID:0000-0002-0651-717X]

Department of Biomedical Engineering, University of Massachusetts Amherst