Data for weight, bowel outcomes, bowel histology, and food intake in female mice after T3 complete transection injury

DOI:10.34945/F57K5H

DATASET CITATION

Wireman O. H., Sams E. L., Richey L. E., Hammers G. V., Stewart A. N., Bailey W. M., Patel S. P., Gensel J. C. (2025) Data for weight, bowel outcomes, bowel histology, and food intake in female mice after T3 complete transection injury. Open Data Commons for Spinal Cord Injury. ODC-SCI:1279 http://doi.org/10.34945/F57K5H

ABSTRACT

STUDY PURPOSE: The goal of this study was to evaluate bowel outcomes in young (4-6 months old) female C57/BL/6J mice after complete T3 transection spinal cord injury

DATA COLLECTED: Sham (laminectomy) or T3 transection: evaluations up to 21 days post spinal cord injury (SCI) including: Food (caloric) and water intake daily alongside body weight measures Fecal output over 1 hour of recording Colon fecal pellet counts at time of euthanasia Bead expulsion from distal colon Anatomical analyses of colon including collagen staining Myography of colon tissue at time of euthansia

CONCLUSIONS: T3 transection SCI in female mice causes weight loss and impairs bowel function as indicated on several outcome measures. The number of fecal pellets retained in the colon increased significantly in animals receiving SCI compared to sham (laminectomy only) injuries by 4 days post-injury (dpi) and persisted to 7 and 21 dpi. Furthermore, the number of pellets expelled (fecal output) significantly decreased in SCI versus sham animals at both 7 and 20 dpi. Pellet size was significantly decreased in the SCI versus sham animals at both 7 and 14 dpi, collectively indicative of decreased motility with SCI. Consistent with these observations, colonic motility using the bead expulsion assay was reduced, non-significantly, in SCI vs. sham animals at all three timepoints. Through ex vivo analyses of live colon sections, we tested the maximal contractility of the circular musculature from both the proximal and distal colon using a myograph system. The maximal contractility of the distal colon significantly increased in SCI compared to sham at 21 dpi. Interestingly, the distal colon of SCI animals at the same time point, 21 dpi, displayed significant collagen deposition in the musculature. These observations are consistent with previous reports in rats and have translational correlates in humans.

KEYWORDS

bowel; dysfunction; constipation; transection; SCI

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RELEVANT LINKS

NOTES

DATASET INFO

Contact: Gensel John (gensel.1@uky.edu)

Lab: Gensel Lab (Nero)

ODC-SCI Accession:1279

Records in Dataset: 61

Fields per Record: 39

Last updated: 2025-02-20

Date published: 2025-02-20

Downloads: 4

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

National Institute of General Medical Science grants GM118292(OHW) and P30 GM127211, National Institute of Neurological Disorders and Stroke T32 NS077889 (OHW), University of Kentucky Spinal Cord and Brain Injury Research Center Endowed Chair #5 (JCG)

CONTRIBUTORS

Wireman, Olivia H. [ORCID:0000-0002-5799-6561]

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Sams, Ellie L.

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Richey, Lynnet E.

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Hammers, Gabrielle V.

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Stewart, Andrew N.

Spinal Cord and Brain Injury Research Center, Neuroscience, University of Kentucky College of Medicine

Bailey, William M.

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Patel, Samir P.

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine

Gensel, John C. [ORCID:0000-0001-8980-108X]

Spinal Cord and Brain Injury Research Center, Physiology, University of Kentucky College of Medicine