Minocycline treatment for acute cervical spinal cord injury in female rats: microbiota composition

DOI:10.34945/F5F30N

DATASET CITATION

Schmidt E. K A., Raposo P. J F., Torres-Espin A., Fenrich K. K., Fouad K. (2021) Minocycline treatment for acute cervical spinal cord injury in female rats: microbiota composition. Open Data Commons for Spinal Cord Injury. ODC-SCI: 454 http://doi.org/10.34945/F5F30N

ABSTRACT

STUDY PURPOSE: Minocycline has been widely studied for its local anti-inflammatory properties; however little is known about its antibiotic and systemic immune effects following spinal cord injury (SCI). The aim of the present study is to elucidate multiple system-wide changes involving the microbiota-immune axis induced by minocycline treatment in a rodent model of cervical contusion SCI (unilateral contusion 125 kdyns at an angle of 15 degrees to the right at C5). This study included 4 groups of adult female Lewis rats: uninjured n=10, uninjured + minocycline n=10, SCI n=8, SCI + minocycline n=9. Minocycline (or sterile water for untreated control groups) was administered at a dose of 50mg/kg via oral gavage for 7 days immediately following injury.

DATA COLLECTED: fecal microbiota data: 16s rRNA sequencing data (at the phylum, class, order, family, genus and species taxonomic levels) from fecal samples collected before injury, on the day of injury, 5, 14 and 28 days post injury

DATA USAGE NOTES: The present data shows that 7 days of minocycline treatment had a drastic acute effect on the microbiota composition regardless of whether the animal was uninjured or had a SCI. Later effects of SCI vs. uninjured rats emerged by 28 days post-SCI.

KEYWORDS

Spinal Cord Injury; minocycline; 16S rRNA

PROVENANCE / ORIGINATING PUBLICATIONS

DATASET INFO

Contact: Fouad Karim (karim.fouad@ualberta.ca)

Lab: Karim Fouad

ODC-SCI Accession:454

Records in Dataset: 180

Fields per Record: 261

Last updated: 2021-01-22

Date published: 2021-01-22

Downloads: 19

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

Craig Neilsen Foundation NPRG 542589 (KF)

CONTRIBUTORS

Schmidt, Emma K A. [ORCID:0000-0001-9803-6391]

Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada

Raposo, Pamela J F. [ORCID:0000-0001-6350-5223]

Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada

Torres-Espin, Abel [ORCID:0000-0002-9787-8738]

University of California San Francisco, Brain and Spinal Injury Center, Dept. of Neurological Surgery; San Francisco, California, USA

Fenrich, Keith K. [ORCID:0000-0003-4360-064X]

Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada

Fouad, Karim [ORCID:0000-0003-3654-7852]

Faculty of Rehabilitation Medicine, University of Alberta; Edmonton, Alberta, Canada