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Probiotic treatment in female Lewis rats following unilateral incomplete cervical (C5) contusion injury: Microbiome reportDOI:10.34945/F5CP4DDATASET CITATIONRaposo P., Nguyen A. T., Schmidt E. K.A., Torres Espin A., Fenrich K. K., Bennett D. J., Fouad K. (2023) Probiotic treatment in female Lewis rats following unilateral incomplete cervical (C5) contusion injury: Microbiome report. Open Data Commons for Spinal Cord Injury. ODC-SCI:688 http://doi.org/10.34945/F5CP4DABSTRACTSTUDY PURPOSE: The bidirectional communication between the gastrointestinal tract and the central nervous system appears to be linked to the intestinal microbiome. Research has shown that spinal cord injury (SCI) can disrupt the gut microbiome, leading to gut dysbiosis. These changes can have several negative impacts, such as exacerbated systemic inflammation and susceptibility to infection. Probiotics administered to mice with SCI have been shown to ameliorate gut dysbiosis, confer neuroprotection, and improve locomotor recovery. However, probiotics have also produced conflicting results, making potential claims regarding the application of probiotics as a therapeutic supplement ambiguous. This study aimed to investigate the effects of a commercially available probiotic on recovery in a rat model of cervical SCI.DATA COLLECTED: Female Lewis rats (12 weeks and 180-220 g) received a C4-C5 laminectomy to expose the spinal cord. Using an Infinite Horizon impactor a 125 kdyn unilateral contusion was performed targeting the right side of the spinal C5 level (1.25 mm right of midline, 25 mm drop height at a 15-degree angle). Probiotic treatment was initiated immediately after SCI using VSL#3 containing 450 billion colony-forming units (CFU) of lyophilized bacteria/g, including eight different strains (Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus planatarum, and Streptococcus salivarus subspecies thermophilus). VSL#3 was administered daily via oral gavage for 7 days postinjury (DPI) at a dose of 5 billion CFU. Control group was gavaged with 0.5 ml of sterile filtered water. After 7 DPI, the 5 billion CFU of VSL#3 doses were adjusted to ensure equivalent consumption and administered ad libitum in drinking water for a total of 35 DPI. The overall gut microbiome was assessed prior to injury (baseline) and at 3, 7, 14, and 35 DPI by collecting fresh fecal matter from the rats during their dark cycle and submitted for 16S rRNA analysis. Dataset for Probiotic treatment for acute spinal cord injury: behavioural, histological and systemic cytokine profiles of female Lewis rats is in OSC-SCI#687CONCLUSIONS: We could not detect any beneficial effects of the probiotic treatment. If anything it was detrimental as lesion size was slightly larger and the return of the gut microbiome to preinjury state was delayed by the treatment. Our results thus do not support the application of the utilized probiotic cocktail as a dietary supplement for the treatment of cervical SCI-induced gut dysbiosis and secondary complications.KEYWORDSSpinal Cord Injury, gut microbiome, motor recovery, Anxiety, rat, probioticsPROVENANCE / ORIGINATING PUBLICATIONSRELEVANT LINKSNOTESDataset for Probiotic treatment for acute spinal cord injury: behavioural, histological and systemic cytokine profiles of female Lewis rats is in ODC-SCI#687
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DATASET INFOContact: Fouad Karim (kfouad@ualberta.ca)Lab: Karim Fouad
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