CatWalk Gait parameters at 8 weeks after T10 thoracic contusion using the MASCIS impactor model III in male and female 8- to 10-week old rats

DOI:10.34945/F5VS3D

DATASET CITATION

Liu S., Xing C., Wei H., Guo J., Wang L., Li B., Ma H., Zhong H., Zhou M., Zhu S., Zhu R., Ning G. (2022) CatWalk Gait parameters at 8 weeks after T10 thoracic contusion using the MASCIS impactor model III in male and female 8- to 10-week old rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:822 http://doi.org/10.34945/F5VS3D

ABSTRACT

STUDY PURPOSE: In preclinical studies of spinal cord injury (SCI), behavioral assessment is crucial in determining the effectiveness of interventions. As an automated gait analysis system, catwalk provides a large number of objective gait parameters through the acquisition and calculation of rodent footprints and videos. However, the gait parameters may not all be suitable for assessing hindlimb locomotor function in animals with thoracic contusion SCI. The purpose of this study was to discover CatWalk gait parameters applicable to different severity ranges (sham, mild, moderate, severe) of T10 spinal cord contusion injury in rats.

DATA COLLECTED: A total of 48 adult male and female Wistar rats, weighing 190-210 g and aged 8-10 weeks old, were used in this study. The thoracic spinal cord of rats was contused by MASCIS impactor model III (W. M. Keck Center, Rutgers University, United States) according to the modified Allen's method. The animals subsequently underwent T10 spinal cord contusions caused by a 10 g impactor dropped at varying heights of 12.5 mm (mild), 25.0 mm (moderate), and 50.0 mm (severe). Animals in the sham group only received laminectomy without contusion.N=12/group. Catwalk gait analysis were performed at 8 weeks post-injury. The performance of moving in one direction without stopping or turning around was recorded.The specific detection settings used in the experiment were as follows: camera gain = 17.75 dB, green intensity threshold = 0.12, red ceiling light= 17.1 V, and green walkway light = 15.4 V. Multiple groups were compared using Kruskal-Wallis test with Dunn's post-hoc test.

CONCLUSIONS: Among the CatWalk gait parameters, regularity index could reflect differences between the Sham, SCI-mil, and SCI-mod groups, but not between the SCI-mod and SCI-sev groups . Our work provides a comprehensively CatWalk gait parameters for SCI studies, which could be applicable to objectively reflect locomotor recovery of T10 spinal cord contusion injury in rats.

KEYWORDS

CatWalk XT; thoracic spinal cord contusion; behavioral testing; locomotor function assessment

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RELEVANT LINKS

NOTES

DATASET INFO

Contact: Ning Guangzhi (gzning@tmu.edu.cn)

Lab: Tianjin Key Laboratory of Spine and Spinal Cord

ODC-SCI Accession:822

Records in Dataset: 140

Fields per Record: 58

Last updated: 2022-12-14

Date published: 2022-12-14

Downloads: 13

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

National Natural Science Foundation of China (82072439) Guangzhi Ning

CONTRIBUTORS

Liu, Song

Tianjin Medical University General Hospital

Xing, Cong

Tianjin Medical University General Hospital

Wei, Haitao

Tianjin Medical University General Hospital

Guo, Junrui

Tianjin Medical University General Hospital

Wang, Liyue

Tianjin Medical University General Hospital

Li, Baicao

Tianjin Medical University General Hospital

Ma, Hongpeng

Tianjin Medical University General Hospital

Zhong, Hao

Tianjin Medical University General Hospital

Zhou, Mi

Tianjin Medical University General Hospital

Zhu, Shibo

Tianjin Medical University General Hospital

Zhu, Rusen

Tianjin Medical University General Hospital

Ning, Guangzhi

Tianjin Medical University General Hospital