Histological evaluation of tissue hypoxia following an unilateral C5 contusion injury in female Long Evans rats

DOI:10.34945/F5XG62

DATASET CITATION

Harmon J. N., Hyde J. E., Jensen D. E., D’cessare E. C., Odarenko A. A., Bruce M. F., Khaing Z. Z. (2024) Histological evaluation of tissue hypoxia following an unilateral C5 contusion injury in female Long Evans rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:890 http://doi.org/10.34945/F5XG62

ABSTRACT

STUDY PURPOSE: Spinal cord injury (SCI) leads to significant perturbations in blood flow in and around the injury epicenter. Over time, these impairments can lead to ischemia and cell death. The purpose of this study is to use intravital contrast-enhanced ultrasound imaging to examine the evaluation of the unilateral cervical SCI using longitudinal imaging to quantify anatomical and hemodynamic changes in vivo through the entire spinal parenchyma.

DATA COLLECTED: Spinal cord samples were collected at 4, 24 or 72 hours post injury (hpi) in three cohorts of animals, and we used a histological method that utilized HypoxyProbe to determine the spatial and temporal distribution of cells that are undergoing hypoxic cell death. In this data set, spatial distribution of the HypoxyProbe staining at 4, 24 and 72 hpi are included. We found that tissue hypoxia was elevated by 4 hpi, and that this remained true at 72 hpi.

CONCLUSIONS: This study highlights the unique advantages of longitudinal ultrasound imaging as a method for in-vivo monitoring of spinal injury evolution. All ultrasound findings were validated histologically.

KEYWORDS

PROVENANCE / ORIGINATING PUBLICATIONS

• . doi:10.1016/j.expneurol.2024.114681.

RELEVANT LINKS

NOTES

DATASET INFO

Contact: Khaing Zin (zink@uw.edu)

Lab: Khaing Lab

ODC-SCI Accession:890

Records in Dataset: 432

Fields per Record: 20

Last updated: 2024-05-16

Date published: 2024-05-16

Downloads: 8

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

NIH NINDS R01NS121191 (ZZK), NIH NICHD F32HD107806 (JNH)

CONTRIBUTORS

Harmon, Jennifer N.

University of Washington

Hyde, Jeffrey E.

University of Washington

Jensen, Dylan E.

University of Washington

D’cessare, Emma C.

University of Washington

Odarenko, Anton A.

University of Washington

Bruce, Matthew F.

University of Washington

Khaing, Zin Z. [ORCID:0000-0001-5652-5891]

University of Washington