• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

 
Full Data Package
 
Data Dictionary
 
Methodology

Create new odc-sci Account and/or to download the file.

Histological evaluation of vascular coverage, apoptosis and tissue sparing following an unilateral C5 contusion injury in female Long Evans rats


DOI:10.34945/F5P015


DATASET CITATION

Harmon J. N., Hyde J. E., Jensen D. E., D’cessare E. C., Odarenko A. A., Bruce M. F., Khaing Z. Z. (2024) Histological evaluation of vascular coverage, apoptosis and tissue sparing following an unilateral C5 contusion injury in female Long Evans rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:894 http://doi.org/10.34945/F5P015


ABSTRACT

STUDY PURPOSE: Spinal cord injury (SCI) leads to significant perturbations in blood flow in and around the injury epicenter. Over time, these impairments can lead to ischemia and cell death. The purpose of this study is to use intravital contrast-enhanced ultrasound imaging to examine the evaluation of the unilateral cervical SCI using longitudinal imaging to quantify anatomical and hemodynamic changes in vivo through the entire spinal parenchyma. In order to histologically characterize the injury, we performed stainings to assess Vascular coverage, apoptosis, and tissue sparing.

DATA COLLECTED: In order to assess tissue changes that occurred as a result of injury, spinal tissue was collected from animals at each timepoint in the study (4, 24, and 72 hpi). Cresyl Violet and Eriochrome staining was performed to quantify tissue sparing. Caspace 8 staining was performed in order to assess the spatial and temporal distribution of apoptotic cell death after injury. We found that tissue sparing was significantly reduced in the 4hpi and 24hpi conditions within the lesion center and the tissue caudal to the lesion center. Tissue loss was found to occur throughout the entire 72 hour window of measurement, with this being especially high in areas rostral to the injury site. In addition to the observed loss of tissue, we noted a substantial increase in apoptotic cell counts at the 24hpi timepoint, an effect that remained true at 72hpi.

CONCLUSIONS: This study highlights the unique advantages of longitudinal ultrasound imaging as a method for in-vivo monitoring of spinal injury evolution. All ultrasound findings were validated histologically.


KEYWORDS


PROVENANCE / ORIGINATING PUBLICATIONS

RELEVANT LINKS


NOTES

DATASET INFO

Contact: Khaing Zin (zink@uw.edu)


Lab: Khaing Lab

ODC-SCI Accession:894

Records in Dataset: 216

Fields per Record: 23

Last updated: 2024-05-16

Date published: 2024-05-16

Downloads: 12


Files: 2


LICENSE

Creative Commons Attribution License (CC-BY 4.0)


FUNDING AND ACKNOWLEDGEMENTS

NIH NINDS R01NS121191 (ZZK), NIH NICHD F32HD107806 (JNH)


CONTRIBUTORS

Harmon, Jennifer N.
University of Washington
Hyde, Jeffrey E.
University of Washington
Jensen, Dylan E.
University of Washington
D’cessare, Emma C.
University of Washington
Odarenko, Anton A.
University of Washington
Bruce, Matthew F.
University of Washington
Khaing, Zin Z. [ORCID:0000-0001-5652-5891]
University of Washington