Full Data Package |
Data Dictionary |
Methodology |
||||
---|---|---|---|---|---|---|
Create new odc-sci Account and/or log in to download the file. |
Histological evaluation of vascular coverage, apoptosis and tissue sparing following an unilateral C5 contusion injury in female Long Evans ratsDOI:10.34945/F5P015DATASET CITATIONHarmon J. N., Hyde J. E., Jensen D. E., D’cessare E. C., Odarenko A. A., Bruce M. F., Khaing Z. Z. (2024) Histological evaluation of vascular coverage, apoptosis and tissue sparing following an unilateral C5 contusion injury in female Long Evans rats. Open Data Commons for Spinal Cord Injury. ODC-SCI:894 http://doi.org/10.34945/F5P015ABSTRACTSTUDY PURPOSE: Spinal cord injury (SCI) leads to significant perturbations in blood flow in and around the injury epicenter. Over time, these impairments can lead to ischemia and cell death. The purpose of this study is to use intravital contrast-enhanced ultrasound imaging to examine the evaluation of the unilateral cervical SCI using longitudinal imaging to quantify anatomical and hemodynamic changes in vivo through the entire spinal parenchyma. In order to histologically characterize the injury, we performed stainings to assess Vascular coverage, apoptosis, and tissue sparing.DATA COLLECTED: In order to assess tissue changes that occurred as a result of injury, spinal tissue was collected from animals at each timepoint in the study (4, 24, and 72 hpi). Cresyl Violet and Eriochrome staining was performed to quantify tissue sparing. Caspace 8 staining was performed in order to assess the spatial and temporal distribution of apoptotic cell death after injury. We found that tissue sparing was significantly reduced in the 4hpi and 24hpi conditions within the lesion center and the tissue caudal to the lesion center. Tissue loss was found to occur throughout the entire 72 hour window of measurement, with this being especially high in areas rostral to the injury site. In addition to the observed loss of tissue, we noted a substantial increase in apoptotic cell counts at the 24hpi timepoint, an effect that remained true at 72hpi.CONCLUSIONS: This study highlights the unique advantages of longitudinal ultrasound imaging as a method for in-vivo monitoring of spinal injury evolution. All ultrasound findings were validated histologically.KEYWORDSPROVENANCE / ORIGINATING PUBLICATIONSRELEVANT LINKSNOTES |
DATASET INFOContact: Khaing Zin (zink@uw.edu)Lab: Khaing Lab
|
|